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Poster De Conférence Année : 2019

High-density genotyping of pea and faba bean diversity panels using exome capture

Gregoire Aubert
Magalie Leveugle
  • Fonction : Auteur
Hervé Duborjal
  • Fonction : Auteur
Jonathan Kreplak
Catherine Desmetz
  • Fonction : Auteur
  • PersonId : 1204367
E. Vieille
  • Fonction : Auteur
Anthony Klein
  • Fonction : Auteur
  • PersonId : 1204654
Marianne Chabert-Martinello
  • Fonction : Auteur
  • PersonId : 1205200
J.P. Pichon
  • Fonction : Auteur
Judith Burstin

Résumé

Genome-wide association studies (GWAS) represent a powerful tool to decipher the genetic determinism of complex traits in crop plants and to identify responsible genes. As GWAS require large diversity panels segregating for the traits of interest, one faba bean and three pea collections were constituted to address different questions in the PeaMUST project. The faba bean panel comprises 248 accessions coming from five continents and displaying the phenotypic diversity of the species. The first pea collection includes 239 accessions that represent the species diversity for aerial and root architecture and for biotic and abiotic stress responses. The second and third pea panels were constituted with 300 accessions of cultivated spring type, 396 accessions of winter type, accessions (376 conventional, hr, 20 Hr) respectively. To generate a large number of unbiased genetic markers, exome capture [1] was performed by taking advantage of (i) transcriptome sequence resources available for pea [2,3], and (ii) newly developed transcriptome for faba bean. The designed probes allowed capturing over 33,000 transcripts in faba bean and 50,000 transcripts in pea. In total, 1.7 and 2.3 million high-quality SNPs have been identified for faba bean and pea, respectively. These markers have been used to assess the genetic structure of the different panels, to perform GWAS for traits of agricultural interest and to design genotyping by capture experiments to undertake genomic selection [4]. These SNP resources will serve to design genotyping arrays in both crops for further experiments.
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Dates et versions

hal-02927852 , version 1 (02-09-2020)

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  • HAL Id : hal-02927852 , version 1

Citer

Gregoire Aubert, Magalie Leveugle, Hervé Duborjal, Nadim N. Tayeh, Jonathan Kreplak, et al.. High-density genotyping of pea and faba bean diversity panels using exome capture. 9th International Conference on Legume Genetics and Genomics (ICLGG 2019), Let's harness the potential of legumes, May 2019, Dijon, France. . ⟨hal-02927852⟩
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