Article Dans Une Revue Chemical Science Année : 2020

A near-infrared fluorogenic dimer enables background-free imaging of endogenous GPCRs in living mice

Lucie Esteoulle
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Mayeul Collot

Résumé

Fluorescent probes are commonly used in studying G protein-coupled receptors in living cells; however their application to the whole animal receptor imaging is still challenging. To address this problem, we report the design and the synthesis of the first near-infrared emitting fluorogenic dimer with environment-sensitive folding. Due to the formation of non-fluorescent H-aggregates in an aqueous medium, the near-infrared fluorogenic dimer displays a strong turn-on response (up to 140-fold) in an apolar environment and exceptional brightness: 56% quantum yield and ≈444 000 M−1 cm−1 extinction coefficient. Grafted on a ligand of the oxytocin receptor, it allows the unprecedented background-free and target-specific imaging of the naturally expressed receptor in living mice.

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hal-02922372 , version 1 (19-11-2020)

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Lucie Esteoulle, François Daubeuf, Mayeul Collot, Stéphanie Riché, Thierry Durroux, et al.. A near-infrared fluorogenic dimer enables background-free imaging of endogenous GPCRs in living mice. Chemical Science, 2020, 11 (26), pp.6824-6829. ⟨10.1039/d0sc01018a⟩. ⟨hal-02922372⟩
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