BACKGROUND: Bean is a rich source of phosphatidylcholine (PC). This study aims to explore natural PC sources rich in polyunsaturated fatty acid (PUFA) with nutritional interest. PCs from six beans were purified (purity > 98.2%) by thin layer chromatography, and subsequently identified by UHPLC-Q-Exactive Orbitrap/MS. RESULTS: Results showed that chickpea and soybean contained the highest quantity of PC among the six beans, making up 50.0 mg/g and 34.0 mg/g, respectively. Gas chromatographic analysis showed that soybean fatty acids contained high proportion of polyunsaturated fatty acid (58.78%), and chickpea contained high proportion of DHA (22:6, 2.73%). A total of 49 molecular species were identified by UHPLC-Q-Exactive Orbitrap/MS. (18:2-18:2)PC was predominant in soybean, red bean, red kidney bean and white kidney bean. (16:0-18:1)PC was the major species of chickpea PC and many plasmanyl-PC species and DHA (22:6)-PC were identified. The Principal Component Analysis (PCA) analysis and hierarchical cluster analysis indicated that the molecular profiles of chickpea PC were significantly different compared to other beans studied. CONCLUSION: The findings suggest that chickpea appears to be an interesting plant source of DHA and ether lipids for dietary supplement. Keywords: Bean PC, UHPLC-Q-Exactive Orbitrap/MS, Molecular species, Identification, PCA