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Communication Dans Un Congrès Année : 2019

Influence of pea genotype on root associated fluorescent pseudomonads, impact on plant iron nutrition

Tristan Lurthy
Cécile Cantat
  • Fonction : Auteur
  • PersonId : 1072032
Catherine Barraud
  • Fonction : Auteur
  • PersonId : 1206845
Sylvie Mazurier

Résumé

Pea has a high potential in agroecology because of its ability to fix atmospheric nitrogen and for Human nutrition due to the high amino-acids content of its seeds. However, pea can suffer from a susceptibility to iron deficiency in calcareous soils as expressed by chlorosis symptoms. Previous studies have shown that siderophores of model strains of fluorescent pseudomonads (fp), pyoverdines, promote iron nutrition of arabidopsis and tobacco. We hypothesized that susceptibility to iron deficiency of pea is at least partly due to its ability to select fluorescent pseudomonad that promote differentially plant nutrition thanks to their siderophores.To identify siderophores potentially beneficial for plant nutrition and, in fine, identify plant genotypes capable of making the most of the rhizosphere microbiota, our strategy is to evaluate (i) influence of the pea genotype on diversity of the rhizosphere microbiota with a special focus on fp and their corresponding pyoverdines and in return ii) the impact of representatives of these pyoverdines on plant iron nutrition.Two distinct pea cultivars chosen for their contrasting capabilities to overcome iron deficiency (S, susceptible and T, tolerant) were grown in a calcareous soil. fp associated with roots of both plant genotypes and corresponding pyoverdines were compared. In return, representative pyoverdines synthesized by fp associated to S and T were compared for their effect on the transcription of genes involved in plant iron uptake and homeostasis (rt-qPCR) and their impact on plant iron content and ionome (HRICP-MS).Differences were observed in the culturable populations of fp associated with S and T. Study of the effect of corresponding predominant pyoverdines showed that they impact differentially plant gene expression and ionome. The results obtained evidence a plant-genotype dependent effect of microbial siderophores on plant nutrition. Further studies are in progress to unravel the mechanisms implied.
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Dates et versions

hal-02909116 , version 1 (30-07-2020)

Identifiants

  • HAL Id : hal-02909116 , version 1

Citer

Tristan Lurthy, Cécile Cantat, Laure Avoscan, Jean-Paul Lemaitre, Catherine Barraud, et al.. Influence of pea genotype on root associated fluorescent pseudomonads, impact on plant iron nutrition. IXe Colloque de l'Association Francophone d'Ecologie Microbienne, AFEM 2019, Fonctions et diversité des microbiotes dans les environnements naturels et anthropisés, Nov 2019, Bussang, France. ⟨hal-02909116⟩
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