Article Dans Une Revue Biochimica et Biophysica Acta - Molecular Cell Research Année : 2014

The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells

Résumé

MUC1 is a membrane-bound mucin known to participate in tumor proliferation. It has been shown that MUC1 pattern of expression is modified during esophageal carcinogenesis, with a progressive increase from metaplasia to adenocarcinoma. The principal cause of development of esophageal adenocarcinoma is gastro-esophageal re-flux and MUC1 was previously shown to be up-regulated by several bile acids present in reflux. In this report, our aim was thus to determine whether MUC1 plays a role in biological properties of human esophageal cancer cells. For that, a stable MUC1-deficient esophageal cancer cell line was established using a shRNA approach. In vitro (proliferation, migration and invasion) and in vivo (tumor growth following subcutaneous xenografts in SCID mice) biological properties of MUC1-deficient cells were analyzed. Our results show that esophageal cancer cells lacking MUC1 were less proliferative and had decreased migration and invasion properties. These alterations were accompanied by a decreased activity of NFKB p65, Akt and MAPK (p44/42, JNK and p38) pathways. MCM6 and TSG101 tumor-associated markers were also decreased. Subcutaneous xenografts showed a significant decrease in tumor size when cells did not express MUC1. Altogether, the data indicate that MUC1 plays a key role in proliferative, migrating and invasive properties of esophageal cancer cells as well as in tumor growth promotion. MUC1 mucin appears thus as a good therapeutic target to slow down esophageal tumor progression.

Fichier principal
Vignette du fichier
1-s2.0-S0167488914002304-main.pdf (738.8 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence
Loading...

Dates et versions

hal-02905652 , version 1 (29-09-2020)

Licence

Identifiants

Citer

Caroline Gronnier, Emilie Bruyère, Fatima Lahdaoui, Nicolas Jonckheere, Michael Perrais, et al.. The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells. Biochimica et Biophysica Acta - Molecular Cell Research, 2014, ⟨10.1016/j.bbamcr.2014.06.021⟩. ⟨hal-02905652⟩

Collections

78 Consultations
276 Téléchargements

Altmetric

Partager

  • More