Biofilm Formation and Quantification Using the 96-Microtiter Plate - Archive ouverte HAL
Chapitre D'ouvrage Année : 2020

Biofilm Formation and Quantification Using the 96-Microtiter Plate

Résumé

Biofilm formation in microtiter plates is certainly the most commonly used method to grow and study biofilm. This simple design is very popular due to its high-throughput screening capacities, low cost, and easy handling. In the protocol described here, we focus on the use of 96-well optically clear, polystyrene flat-bottom plate to study biofilm formation by Leptospira spp. and quantify the biofilm formation by crystal violet (CV) staining. We also describe an alternative method, based on phase contrast image analysis that we believe is more suitable for accurately quantifying biofilm growth by reducing handling of this fragile structure.
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Dates et versions

hal-02899284 , version 1 (15-07-2020)

Identifiants

Citer

Roman Thibeaux, Malia Kainiu, Cyrille Goarant. Biofilm Formation and Quantification Using the 96-Microtiter Plate. Springer. Leptospira spp.: Methods and Protocols, 2134, Humana, pp.207-214, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_19⟩. ⟨hal-02899284⟩
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