Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Peptide Science Année : 2020

Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications

Résumé

The development of tailorable and biocompatible three-dimensional (3D) substrates or molecular networks that reliably mimic the extracellular matrix (ECM) and influence cell behavior and growth in vitro is of increasing interest for cell-based applications in the field of tissue engineering and regenerative medicine. In this context, we present a novel coiled coilbased peptide that self-assembles into a 3D-$\alpha$-helical fibril network and functions as a selfsupporting hydrogel. By functionalizing distinct coiled-coil peptides with cellular binding motifs (RGD) or carbohydrate ligands (mannose), and by utilizing the multivalency and modularity of coiled-coil assemblies, tailored artificial ECMs are obtained. Fibrillar network and ligand density, as well as ligand composition can readily be adjusted by changes in water content or peptide concentrations, respectively. Mesoscopic structure of these networks was assessed by rheology and small-angle neutron scattering experiments. Initial cell viability studies using NIH/3T3 cells showed comparable or even superior cell viability using the presented artificial ECMs, compared to commercially available 3D-cell culture scaffold Matrigel®. The herein reported approach presents a reliable (low batch-to-batch variation) and modular pathway towards biocompatible and tailored artificial ECMs.

Domaines

Polymères
Fichier principal
Vignette du fichier
Manuscript_Hydrogels_final_01072020_BL_KH.pdf (3.18 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02893349 , version 1 (08-07-2020)

Licence

Identifiants

Citer

Katharina S. Hellmund, Benjamin Von Lospichl, Christoph Böttcher, Kai Ludwig, Uwe Keiderling, et al.. Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications. Peptide Science, 2020, 113 (2), ⟨10.1002/pep2.24201⟩. ⟨hal-02893349⟩
214 Consultations
77 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More