Interactions between amyloid precursor protein-like (APPL) and MAGUK scaffolding proteins contribute to appetitive long-term memory in Drosophila melanogaster - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Neurogenetics Année : 2020

Interactions between amyloid precursor protein-like (APPL) and MAGUK scaffolding proteins contribute to appetitive long-term memory in Drosophila melanogaster

Résumé

Amyloid precursor protein (APP), the precursor of amyloid beta peptide, plays a central role in Alzheimer's disease (AD), a pathology characterized by memory decline and synaptic loss upon aging. Understanding the physiological role of APP is fundamental in deciphering the progression of AD, and several studies suggest a synaptic function via protein-protein interactions. Nevertheless, it remains unclear whether and how these interactions contribute to memory. In Drosophila, we previously showed that APP-like (APPL), the fly APP homolog, is required for aversive associative memory in the olfactory memory center, the mushroom body (MB). In the present study, we show that APPL is required for appetitive long-term memory (LTM), another form of associative memory, in a specific neuronal subpopulation of the MB, the a 0 /b 0 Kenyon cells. Using a biochemical approach, we identify the synaptic MAGUK (membrane-associated guanylate kinase) proteins X11, CASK, Dlgh2 and Dlgh4 as interactants of the APP intracellular domain (AICD). Next, we show that the Drosophila homologs CASK and Dlg are also required for appetitive LTM in the a 0 /b 0 neurons. Finally, using a double RNAi approach, we demonstrate that genetic interactions between APPL and CASK, as well as between APPL and Dlg, are critical for appetitive LTM. In summary, our results suggest that APPL contributes to asso-ciative long-term memory through its interactions with the main synaptic scaffolding proteins CASK and Dlg. This function should be conserved across species.
Fichier principal
Vignette du fichier
SilvaPreat2020 HAL.pdf (4.41 Mo) Télécharger le fichier
Silva et al Supplemental table 1.xlsx (93.71 Ko) Télécharger le fichier
Silva et al supplementary table 2.xlsx (50.97 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02892323 , version 1 (29-07-2020)

Identifiants

Citer

Bryon Silva, Christian Niehage, Marta Maglione, Bernard Hoflack, Stephan J. Sigrist, et al.. Interactions between amyloid precursor protein-like (APPL) and MAGUK scaffolding proteins contribute to appetitive long-term memory in Drosophila melanogaster. Journal of Neurogenetics, 2020, Future Perspectives of Neurogenetics - in honor of Troy D. Zars, 34 (1), pp.92-105. ⟨10.1080/01677063.2020.1712597⟩. ⟨hal-02892323⟩
97 Consultations
291 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More