HepaRG Self-Assembled Spheroids in Alginate Beads Meet the Clinical Needs for Bioartificial Liver - Archive ouverte HAL Access content directly
Journal Articles Tissue Engineering: Parts A, B, and C Year : 2020

HepaRG Self-Assembled Spheroids in Alginate Beads Meet the Clinical Needs for Bioartificial Liver

Abstract

In liver tissue engineering, cell culture in spheroids is now well recognized to promote the maintenance of hepatic functions. However, the process leading to spheroids formation is time consuming, costly, and not easy to scale-up for further use in human bioartificial liver (BAL) applications. In this study, we encapsulated HepaRG cells (precursors of hepatocyte-like cells) in alginate 1.5% without pre-forming spheroids. Starting from a given hepatic biomass, we analysed cell differentiation and metabolic performance for further use in fluidized-bed BAL. We observed that cells rearranged as aggregates into the beads and adequately differentiated over time, in the absence of any differentiating factors classically used. At day 14 post-encapsulation, cells displayed a large set of hepatic features necessary for the treatment of a patient in acute liver failure. These activities include albumin synthesis, ammonia and lactate detoxification and the efficacy of the enzymes involved in the xenobiotic metabolism (such as CYP1A1/2).
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Dates and versions

hal-02884175 , version 1 (11-12-2020)

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Mattia Pasqua, Ulysse Pereira, Antonietta Messina, Claire de Lartigue, Pascale Vigneron, et al.. HepaRG Self-Assembled Spheroids in Alginate Beads Meet the Clinical Needs for Bioartificial Liver. Tissue Engineering: Parts A, B, and C, 2020, 26 (11-12), pp.613-622. ⟨10.1089/ten.tea.2019.0262⟩. ⟨hal-02884175⟩
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