Canalicular domain structure and function in matrix-free hepatic spheroids - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biomaterials Science Année : 2020

Canalicular domain structure and function in matrix-free hepatic spheroids

Résumé

Liver is pivotal in organism metabolism. This organ is receiving nutriments from the portal vein and then storing, metabolizing, distributing in the circulation or excreting excess and xenobiotics in bile. Liver architecture and hepatocyte polarization are crucial to achieve these functions. To study these mechanisms in details, relevant cell culture systems are required, which is not the case with standard 2D cell culture. Besides, primary hepatocytes rapidly de-differenciate making them inefficient in forming physiological system. Herein, we used an hepatoma-derived cell line to produce matrix-free hepatic spheroids and developed an integrated structural cell biology methodology by combining light sheet fluorescence microscopy and 3D electron microscopy to study their function and structure. Within these spheroids, hepatocytes polarize and organize to form bile canaliculi active for both organics and inorganics excretion. Besides, live imaging revealed the high dynamic of actin networks in basal membranes compared to their high stability in the apical pole that constitutes bile canaliculi. Finally, the first structure of active bile canaliculi was solved at nm resolution and showed the very high density of microvilli coming from all cells constituting the canaliculus. Therefore, this study is the first comprehensive and in-depth functional and structural study of bile canaliculi in a physiological-relevant context.
Fichier principal
Vignette du fichier
Manuscrit-auteurs-hal-02873790.pdf (1.81 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02873790 , version 1 (05-11-2021)

Identifiants

Citer

Vikas Raj Sharma, Ananya Shrivastava, Benoit Gallet, Elizaveta Karepina, Peggy Charbonnier, et al.. Canalicular domain structure and function in matrix-free hepatic spheroids. Biomaterials Science, 2020, 8 (1), pp.485-496. ⟨10.1039/c9bm01143a⟩. ⟨hal-02873790⟩
77 Consultations
166 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More