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Journal Articles Journal of Applied Crystallography Year : 2020

Optimization of crystallization of biological macromolecules using dialysis combined with temperature control

Abstract

A rational way to find the appropriate conditions to grow crystal samples for bio-crystallography is to determine the crystallization phase diagram, which allows precise control of the parameters affecting the crystal growth process. First, the nucleation is induced at supersaturated conditions close to the solubility boundary between the nucleation and metastable regions. Then, crystal growth is further achieved in the metastable zone – which is the optimal location for slow and ordered crystal expansion – by modulation of specific physical parameters. Recently, a prototype of an integrated apparatus for the rational optimization of crystal growth by mapping and manipulating temperature–precipitant–concentration phase diagrams has been constructed. Here, it is demonstrated that a thorough knowledge of the phase diagram is vital in any crystallization experiment. The relevance of the selection of the starting position and the kinetic pathway undertaken in controlling most of the final properties of the synthesized crystals is shown. The rational crystallization optimization strategies developed and presented here allow tailoring of crystal size and diffraction quality, significantly reducing the time, effort and amount of expensive protein material required for structure determination.

Dates and versions

hal-02864672 , version 1 (11-06-2020)

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Niels Junius, Elham Vahdatahar, Esko Oksanen, Jean-Luc Ferrer, Monika Budayova-Spano. Optimization of crystallization of biological macromolecules using dialysis combined with temperature control. Journal of Applied Crystallography, 2020, 53 (3), pp.686-698. ⟨10.1107/S1600576720003209⟩. ⟨hal-02864672⟩
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