HS-SPME-GC-MS-based untargeted metabolomics for kinetics tracking of natural herbicides’ volatile residues: can we “footprint” the “Volatilome”?? - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2019

HS-SPME-GC-MS-based untargeted metabolomics for kinetics tracking of natural herbicides’ volatile residues: can we “footprint” the “Volatilome”??

Résumé

Despite their known risks, herbicides are still essential for agriculture. Thus, natural herbicides are increasingly recommended to replace synthetic ones. However, there are still limitations in studying the environmental fate of several of them. The main reason is the lack of methods dedicated for this type of complex bio-herbicides. Hence, in the framework of the Environmental Metabolic Footprinting (EMF) approach, the current work presents a method dedicated to analyze the volatile residues of herbicides applied on soil. The aim is to track the evolution of the volatile compounds issued from the herbicide by an untargeted metabolomics-based kinetics, in order to determine the “resilience time” of the gaseous phase above the soil. The approach aims to explore the environmental fate of these herbicides. In fact, it will supplement other methods based on the EMF approach recently developed. Moreover, it can estimate the exposure of farmers, insects and plants to potential toxic volatile substances. The method is based on Headspace-Solid Phase Micro Extraction-Gas Chromatography-Electronic Impact Ionization-Quadrupole Mass Spectrometry (HS-SPME-GC-EI-Q MS). The HS-SPME provides a non-destructive extraction. This allows reducing the number of samples. The GC separation technique provides high reproducible analysis for volatile compounds. In addition, it allows the calculation of the Retention Index (RI) as a tool for the molecular characterization. On the other hand, despite the low resolution of the Quadrupole mass analyzer, the Electronic Impact Ionization provides reproducible MS fragmentation patterns used for spectral library search and fast putative identification of unknown compounds.
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hal-02706143 , version 1 (01-06-2020)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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  • HAL Id : hal-02706143 , version 1

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Hikmat Ghosson, Delphine Raviglione, Marie-Virginie Salvia, Cedric Bertrand. HS-SPME-GC-MS-based untargeted metabolomics for kinetics tracking of natural herbicides’ volatile residues: can we “footprint” the “Volatilome”??. Journée Métabolomique en Occitanie (R-Oc-Me), Centre de Recherches Insulaires et Observatoire de l’Environnement - CRIOBE USR3278 - EPHE-CNRS-UPVD; Université de Perpignan Via Domitia, Oct 2019, Perpignan, France. pp.1. ⟨hal-02706143⟩
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