Fumonisin B-1 exposure and its selective effect on porcine jejunal segment: Sphingolipids, glycolipids and trans-epithelial passage disturbance - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Pharmacology Année : 2007

Fumonisin B-1 exposure and its selective effect on porcine jejunal segment: Sphingolipids, glycolipids and trans-epithelial passage disturbance

Résumé

Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium verticillioides, the cause of Fusarium kernel rot in maize. FB1 is toxic in domestic and laboratory animals, including pigs. This study investigated the effects of a seven-days-exposure of 1.5 mg/kg b.w. FB1 on the porcine intestinal epithelium. Statistically significant increases in the ratio of sphinganine to sphingosine, as well as alterations of the glycolipid distribution were observed in the jejunum. Using a porcine intestinal epithelial cell line (IPEC-1) derived from jejunum and ileum, we tested the effect of FB1 in vitro in a time- and dose-dependent fashion. A significant increase in sphinganine concentration was observed after 2 days of FB1 exposure at concentrations >100 mM, or from 6 days of FB1 exposure at concentration >20 mM. We were also able to show that FB1 exposure at 200 mM during 16 days increased the intestinal transepithelial flux of FB1. These data indicate that, in pigs, this mycotoxin acts selectively on jejunum cells as follows: (i) FB1 affects sphingolipid metabolism, as demonstrated by an increase of the amount of free sphingoid bases in a time- and dose-dependent manner, (ii) a depletion of the glycolipids in plasma membranes is observed, and (iii) an increase occurs in the trans-epithelial flux.

Dates et versions

hal-02663891 , version 1 (31-05-2020)

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Nicolas Loiseau, Laurent Debrauwer, Tounkang Sambou, Sandrine Bouhet, J. David Millet, et al.. Fumonisin B-1 exposure and its selective effect on porcine jejunal segment: Sphingolipids, glycolipids and trans-epithelial passage disturbance. Biochemical Pharmacology, 2007, 74 (1), pp.144-152. ⟨10.1016/j.bcp.2007.03.031⟩. ⟨hal-02663891⟩
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