Nonenzymatic oxygenated metabolite of docosahexaenoic acid, 4( RS )-4-F 4t -neuroprostane, acts as a bioactive lipid molecule in neuronal cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2020

Nonenzymatic oxygenated metabolite of docosahexaenoic acid, 4( RS )-4-F 4t -neuroprostane, acts as a bioactive lipid molecule in neuronal cells

Résumé

Docosahexaenoic acid (DHA), an abundant fatty acid in the brain, is susceptible to auto-oxidation in situ and releases metabolites such as F4 -neuroprostane (4-F4t -NeuroP). The presence of 4-F4t -NeuroP in the brain is not well explored. In this study, 4-F4t -NeuroP was introduced into neuroblastoma cells (SH-SY5Y) and, by in vivo infusion, into rodents. Targeted lipidomic analysis of liver and brain tissues shows significant elevation of anti-inflammatory hydroxylated DHA metabolites and an isomer of neuroprotectin D1, suggesting potential beneficial bioactivities of 4-F4t -NeuroP. Additionally, 4-F4t -NeuroP treatment in SH-SY5Y cells and primary neuronal culture consistently upregulates the transcriptional level of the antioxidant enzyme heme oxygenase-1, but the effect is reduced when 4-F4t -NeuroP is further oxidized. Our data suggest that 4-F4t -NeuroP could be neuroprotective in the native state but may have disadvantageous bioactivity when oxidized extensively.

Dates et versions

hal-02617530 , version 1 (25-05-2020)

Identifiants

Citer

Yiu Yiu Lee, Jean-Marie Galano, Ho Hang Leung, Laurence Balas, Camille Oger, et al.. Nonenzymatic oxygenated metabolite of docosahexaenoic acid, 4( RS )-4-F 4t -neuroprostane, acts as a bioactive lipid molecule in neuronal cells. FEBS Letters, 2020, 594 (11), pp.1797-1808. ⟨10.1002/1873-3468.13774⟩. ⟨hal-02617530⟩
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