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Article Dans Une Revue Journal of Biomedical Materials Research Année : 2002

The influence of culture conditions on extracellular matrix proteins synthesized by osteoblasts derived from rabbit bone marrow

Francois Cornet
  • Fonction : Auteur
Karine Anselme
Thierry Grard
Myriam Rouahi
Benoit Noël
  • Fonction : Auteur
Pierre Hardouin
  • Fonction : Auteur
Joseph Jeanfils
  • Fonction : Auteur

Résumé

The influence of culture conditions on the extracellular matrix (ECM) protein expressions of rabbit bone marrow stromal cells has been studied. The focus was on the effects of two kinds of sera, fetal calf serum (FCS) and Ultroser®, on cells treated with dexamethasone. The induction of osteoblastic differentiation by dexamethasone addition is confirmed, particularly when cells are cultured in FCS. Bone marrow stromal cells produce alkaline phosphatase positive CFU‐F and produce ECM with some mineralized nodules. Analysis by means of two‐dimensional gel electrophoresis showed important changes in the composition of ECM proteins after dexamethasone treatment. Overexpression, underexpression, and new synthesized proteins were observed. The most significant modification was linked to the synthesis of four new proteins visible in the acidic area with a low molecular weight of around 17 kDa. These proteins did not correspond to those ECM proteins known to be induced by dexamethasone. Moreover, the effect of dexamethasone on osteoblastic differentiation induction appears very limited when cells are cultured in Ultroser® compared to FCS. The protein pattern with Ultroser® is different to that obtained with FCS. Cells cultured in Ultroser® synthesized no new protein. The different behavior of cells according to the type of medium used is discussed in terms of the osteogenic factors present in the two different sera.

Dates et versions

hal-02566287 , version 1 (06-05-2020)

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Citer

Francois Cornet, Karine Anselme, Thierry Grard, Myriam Rouahi, Benoit Noël, et al.. The influence of culture conditions on extracellular matrix proteins synthesized by osteoblasts derived from rabbit bone marrow. Journal of Biomedical Materials Research, 2002, 63 (4), pp.400-407. ⟨10.1002/jbm.10252⟩. ⟨hal-02566287⟩
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