Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins. - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 1987

Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins.

A. Dorn
  • Function : Author
C. Marfing
  • Function : Author
M. Le Meur
  • Function : Author
D. Mathis
  • Function : Author

Abstract

A conserved sequence motif exists at the 5' end of all major histocompatibility complex class II genes. This motif consists of the 14-base X and Y boxes separated by a short stretch of variable sequence. In this report, we provide evidence that the X and Y boxes play an important role in controlling transcription of the murine class II gene E kappa alpha. We have developed transgenic mouse lines that carry E alpha genes cleanly deleted for either the X or Y box and have compared the expression of these mutant transgenes with that of a nondeleted control. Both the X and Y segments appear critical for accurate and efficient transcription of E kappa alpha. The most drastic effect is seen with gamma-interferon-treated macrophages, where deletion of the Y box completely abrogates transcription initiated by the normal promoter. In addition, we identify proteins from nuclear extracts that bind specifically to the X or Y box.
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hal-02561163 , version 1 (24-11-2020)

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A. Dorn, Beatrice Claude Durand, C. Marfing, M. Le Meur, C. Benoist, et al.. Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins.. Proceedings of the National Academy of Sciences of the United States of America, 1987, 84 (17), pp.6249-6253. ⟨10.1073/pnas.84.17.6249⟩. ⟨hal-02561163⟩
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