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Article Dans Une Revue International Journal of Biological Macromolecules Année : 2018

Thermal restraint of a bacterial exopolysaccharide of shallow vent origin

Résumé

To dynamically characterize the thermal properties of the fructose-rich exopolysaccharide (EPS1-T14), produced by the marine thermophilic Bacillus licheniformis T14, the Attenuated Total Reflectance Fourier Transform Infra-Red spectroscopy was coupled to variable temperature ranging from ambient to 80 °C. The spectra were analyzed by the following innovative mathematical tools: i) non-ideal spectral deviation, ii) OHstretching band frequency center shift, iii) spectral distance, and iv) wavelet cross-correlation analysis. The thermal restraint analysis revealed that the whole EPS1-T14 system possessed high stability until 80 °C, and suggested that fucose was mainly involved in the EPS1-T14 thermal stability, whereas glucose was responsible for its molecular flexibility. Our results provide novel insights into the thermal stability properties of the whole EPS1-T14 and into the role of its main monosaccharidic units. As a new biopolymer, the thermostable EPS1-T14 could be used in traditional biotechnology fields and in new biomedical areas, as nanocarriers, requiring high temperature processes.
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Dates et versions

hal-02557266 , version 1 (28-04-2020)

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Maria Teresa Caccamo, Vincenzo Zammuto, Concetta Gugliandolo, Claire Madeleine-Perdrillat, Antonio Spanò, et al.. Thermal restraint of a bacterial exopolysaccharide of shallow vent origin. International Journal of Biological Macromolecules, 2018, 114, pp.649-655. ⟨10.1016/j.ijbiomac.2018.03.160⟩. ⟨hal-02557266⟩
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