Thermal restraint of a bacterial exopolysaccharide of shallow vent origin
Résumé
To dynamically characterize the thermal properties of the fructose-rich exopolysaccharide (EPS1-T14), produced by the
marine thermophilic Bacillus licheniformis T14, the Attenuated Total Reflectance Fourier Transform Infra-Red
spectroscopy was coupled to variable temperature ranging from ambient to 80 °C.
The spectra were analyzed by the following innovative mathematical tools: i) non-ideal spectral deviation, ii) OHstretching
band frequency center shift, iii) spectral distance, and iv) wavelet cross-correlation analysis.
The thermal restraint analysis revealed that the whole EPS1-T14 system possessed high stability until 80 °C, and
suggested that fucose was mainly involved in the EPS1-T14 thermal stability, whereas glucose was responsible for its
molecular flexibility.
Our results provide novel insights into the thermal stability properties of the whole EPS1-T14 and into the role of its
main monosaccharidic units. As a new biopolymer, the thermostable EPS1-T14 could be used in traditional
biotechnology fields and in new biomedical areas, as nanocarriers, requiring high temperature processes.
Domaines
Sciences du Vivant [q-bio]
Fichier principal
7_International Journal of Biological Macromolecules_A_2018_Magazù.pdf (1.24 Mo)
Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...