Caveolae-mediated effects of TNF-alpha on human skeletal muscle cells - Archive ouverte HAL
Article Dans Une Revue Experimental Cell Research Année : 2018

Caveolae-mediated effects of TNF-alpha on human skeletal muscle cells

Résumé

Chronic diseases are characterized by the production of pro-inflammatory cytokines such than TNF-alpha and are frequently correlated with muscle wasting conditions. Among the pleiotropic effects of TNF-alpha within the cell, its binding to TNFR1 receptor has been shown to activate sphingomyelinases leading to the production of ceramides. Sphingomyelinases and TNF receptor have been localized within caveolae which are specialized RAFT enriched in cholesterol and sphingolipids. Because of their inverted omega shape, maintained by the oligomerization of specialized proteins, caveolins and cavins, caveolae serve as membrane reservoir therefore providing mechanical protection to plasma membranes. Although sphingolipids metabolites, caveolins and TNF-alpha/TNFR1 have been shown to independently interfere with muscle physiology, no data have clearly demonstrated their concerted action on muscle cell regeneration. In this context, our study aimed at studying the molecular mechanisms induced by TNF-alpha at the level of caveolae in LHCN-M2 human muscle satellite cells. Here we showed that TNF-alpha-induced production of ROS and nSMase activation requires caveolin. More strikingly, we have demonstrated that TNF-alpha induces the formation of additional caveolae at the plasma membrane of myoblasts. Furthermore, TNF-alpha prevents myoblast fusion suggesting that inflammation could modulate caveolae organization/function and satellite cell function.

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Matériaux
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Dates et versions

hal-02543571 , version 1 (15-04-2020)

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Elise Dargelos, Valentine Renaud, Marion Decossas, Corinne Bure, Olivier Lambert, et al.. Caveolae-mediated effects of TNF-alpha on human skeletal muscle cells. Experimental Cell Research, In press, 370 (2), pp.623-631. ⟨10.1016/j.yexcr.2018.07.027⟩. ⟨hal-02543571⟩

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