The hydro-distillation of the essential oil of Helichrysum italicum variety numidicum Pomel gave a viscous liquid with pale yellow oil. The average yield of essential oil of the samples is 0.03%. The chemical composition of essential oil was analyzed by GC and GC/MS. A total 43 compounds representing 92.9% of the oil were identified in H. italicum. The chemical composition is dominated by the presence of major products, Isopropyl tetradecanoate (12.10%), α-pinene (12.02%), Hexadecanoic acid (9.96%), Caryophyllene (E) (9.22%), Ledol (9.11%), Palustrol (5.55%), α-Humulene (4.28%), Caryophyllene <9-epi> (3.75%), α-copaene (3.72%), cis-Calamenene (2.18%). The Essential oil of H. italicum was tested for six microorganisms. The oil showed modest effect against all the bacteria tested and it has no significant antifungal activity against the Candida albicans. The population of Helichrysum italicum shows a tertraploïde chromosome number of 2n = 28 with basic number x = 7.