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Journal Articles Biomicrofluidics Year : 2020

The effect of shear stress reduction on endothelial cells: a microfluidic study of the actin cytoskeleton

Abstract

Reduced blood flow, as occurring in ischemia or resulting from exposure to microgravity such as encountered in space flights, induces a decrease in the level of shear stress sensed by the endothelial cells forming the inner part of blood vessels. In the present study, we use a microvasculature-on-a-chip device in order to investigate in vitro the effect of such a reduction in shear stress on shear-adapted endothelial cells. We find that, within one hour of exposition to reduced wall shear stress, human umbilical vein endothelial cells undergo a reorganization of their actin skeleton, with a decrease in the number of stress fibers and actin being recruited into the cells' peripheral band, indicating a fairly fast change in cells' phenotype due to altered flow.
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Dates and versions

hal-02530071 , version 1 (03-04-2020)
hal-02530071 , version 2 (07-04-2020)

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Mehdi Inglebert, Laura Locatelli, Daria Tsvirkun, Priti Sinha, Jeanette A Maier, et al.. The effect of shear stress reduction on endothelial cells: a microfluidic study of the actin cytoskeleton. Biomicrofluidics, 2020, ⟨10.1063/1.5143391⟩. ⟨hal-02530071v2⟩
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