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Article Dans Une Revue Journal of Molecular Microbiology and Biotechnology Année : 2012

Galleria mellonella as aModel for Studying Enterococcus faecium Host Persistence

Résumé

Enterococcus faecium is an opportunistic pathogen responsible for numerous outbreaks worldwide. The basis for the colonization capacities, host persistence and environmental stress response of the hospital-adapted clones emerging from E. faecium are poorly understood. In this study, we propose the use of Galleria mellonella as a simple non mammalian model to assess E. faecium host persistence. Various strains (n = 10), including hospital-adapted, commensal or animal isolates and a SodA-deficient strain were used to assess the relevance of this model. Compared to Enterococcus faecalis, E. faecium strains do not appear very lethal in a Galleria killing assay. The ability of E. faecium strains to overcome host-immune responses and multiply within the host system was evaluated by monitoring bacterial loads following Galleria infection. Among the E. faecium strains, two hospital-adapted isolates displayed increased colonization ability. In contrast, inactivation of sodA, encoding a putative manganese-dependent superoxide dismutase, significantly reduced survival of E. faecium to Galleria defenses. Galleria appears to be a suitable and convenient surrogate model to study E. faecium survival to host defenses and the role of suspected virulence factors in the colonization process. Copyright (C) 2012 S. Karger AG, Basel
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Dates et versions

hal-02528638 , version 1 (01-04-2020)

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François Lebreton, Florian Le Bras, Fany F. Reffuveille, Rabia R. Ladjouzi, Jean-Christophe Giard, et al.. Galleria mellonella as aModel for Studying Enterococcus faecium Host Persistence. Journal of Molecular Microbiology and Biotechnology, 2012, 21 (3-4), pp.191-196. ⟨10.1159/000332737⟩. ⟨hal-02528638⟩
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