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Article Dans Une Revue Brain Structure and Function Année : 2009

The influence of catecholamine on the migration of gonadotropin-releasing hormone-producing neurons in the rat foetuses

Résumé

atecholamines (CA) play an important role inthe regulation of GnRH neurons in adults, and it is probablethat they control GnRH-neuron development. Migration ofGnRH neurons was evaluated in male and female rats at the17th embryonic day (E17) and E21, following the dailytreatment of their pregnant mothers from the 11th to the16th and 20th day of gestation witha-methyl-para-tyrosine(aMPT), an inhibitor of catecholamine synthesis. High-performance liquid chromatography with electrochemicaldetection (HPLC-ED) was used to specify theaMPT-induced CA depletion. There was a 50–70% decrease indopamine and noradrenaline content in the nose and in thebrain ofaMPT-treated foetuses, proving the efficacy of thispharmacological model. Immunohistochemistry was usedto evaluate the percentage (%) of GnRH neurons alongtheir migration pathway from the vomeronasal organ(VNO) in the nose to the septo-preoptic area in the fore-brain which is considered as an index of neuron migration.Special attention was paid to the topographic relationshipsof GnRH neurons with catecholaminergic fibres. Thesewere observed in apposition with GnRH neurons in theentrance to the forebrain. In CA-deficient foetuses, thepercentage of GnRH neurons located in the rostral regionsextending from the VNO to the septum was greater than incontrols. However, no statistically significant differencewas found in the forebrain which extended from the septumto the retrochiasmatic area. In conclusion, these data sug-gest that endogenous catecholamines stimulate the GnRHneuron migration in ontogenesis.

Dates et versions

hal-02527028 , version 1 (31-03-2020)

Identifiants

Citer

M. Izvolskaia, Anne Duittoz, Yves Tillet, M. Ugrumov. The influence of catecholamine on the migration of gonadotropin-releasing hormone-producing neurons in the rat foetuses. Brain Structure and Function, 2009, 213 (3), pp.289-300. ⟨10.1007/s00429-008-0197-x⟩. ⟨hal-02527028⟩
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