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Article Dans Une Revue Journal of Neurophysiology Année : 2015

Optogenetically induced spatiotemporal gamma oscillations and neuronal spiking activity in primate motor cortex

Yao Lu
  • Fonction : Auteur
Wilson Truccolo
  • Fonction : Auteur
Fabien Bertrand Paul Wagner
Ilker Ozden
  • Fonction : Auteur
Travis May
  • Fonction : Auteur
Naubahar Agha
  • Fonction : Auteur
Jing Wang
  • Fonction : Auteur
  • PersonId : 1004603
Arto Nurmikko
  • Fonction : Auteur

Résumé

Transient gamma-band (40–80 Hz) spatiotemporal patterns are hypothesized to play important roles in cortical function. Here we report the direct observation of gamma oscillations as spatiotemporal waves induced by targeted optogenetic stimulation, recorded by intracortical multichannel extracellular techniques in macaque monkeys during their awake resting states. Microelectrode arrays integrating an optical fiber at their center were chronically implanted in primary motor (M1) and ventral premotor (PMv) cortices of two subjects. Targeted brain tissue was transduced with the red-shifted opsin C1V1(T/T). Constant (1-s square pulses) and ramp stimulation induced narrowband gamma oscillations during awake resting states. Recordings across 95 microelectrodes (4 × 4-mm array) enabled us to track the transient gamma spatiotemporal patterns manifested, e.g., as concentric expanding and spiral waves. Gamma oscillations were induced well beyond the light stimulation volume, via network interactions at distal electrode sites, depending on optical power. Despite stimulation-related modulation in spiking rates, neuronal spiking remained highly asynchronous during induced gamma oscillations. In one subject we examined stimulation effects during preparation and execution of a motor task and observed that movement execution largely attenuated optically induced gamma oscillations. Our findings demonstrate that, beyond previously reported induced gamma activity under periodic drive, a prolonged constant stimulus above a certain threshold may carry primate motor cortex network dynamics into gamma oscillations, likely via a Hopf bifurcation. More broadly, the experimental capability in combining microelectrode array recordings and optogenetic stimulation provides an important approach for probing spatiotemporal dynamics in primate cortical networks during various physiological and behavioral conditions.

Domaines

Neurobiologie

Dates et versions

hal-02513125 , version 1 (20-03-2020)

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Citer

Yao Lu, Wilson Truccolo, Fabien Bertrand Paul Wagner, Carlos Vargas-Irwin, Ilker Ozden, et al.. Optogenetically induced spatiotemporal gamma oscillations and neuronal spiking activity in primate motor cortex. Journal of Neurophysiology, 2015, 113 (10), pp.3574-3587. ⟨10.1152/jn.00792.2014⟩. ⟨hal-02513125⟩
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