Real Time and Quantitative Imaging of Lignocellulosic Films Hydrolysis by Atomic Force Microscopy Reveals Lignin Recalcitrance at Nanoscale - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biomacromolecules Année : 2019

Real Time and Quantitative Imaging of Lignocellulosic Films Hydrolysis by Atomic Force Microscopy Reveals Lignin Recalcitrance at Nanoscale

Delphine Dessaint
  • Fonction : Auteur
Laurence Foulon
  • Fonction : Auteur
  • PersonId : 1205734
  • IdRef : 171107845
Brigitte Chabbert
Gabriel Paës

Résumé

Lignocellulosic biomass is considered as a sustainable source of energy and chemicals, but its recalcitrance to bioconversion still limits its use. In this paper, a strategy based on two aspects was developed to improve our knowledge on the lignin recalcitrance to enzymatic hydrolysis. First, lignocellulosic films of cellulose nanofibrils (CNFs) with increasing content of lignin (up to 40%) were prepared. Thanks to in situ real time Atomic Force Microscopy (AFM) measurements during the hydrolysis and by comparison with biochemical assays, the use of such films allows to fully assess the importance of the lignin content and of the arrangement between CNFs and lignin on the hydrolysis efficiency. In a second time, contrary to other studies by AFM which only followed a specific structure during enzymatic processes mostly on simple systems (CNFs or cellulose nanocrystals), a quantitative analysis of in-situ time-lapse measurements was developed. It enables to accurately address lignocellulosic biomass recalcitrance mechanisms mediated by lignin at nanoscale. Such analysis could pave the way for the use of a quantitative criteria to visualize in situ deconstruction of complex lignocellulosic substrates. Coupling the use of lignocellulosic films and dynamical AFM quantitative analysis to follow the evolution of the structure at nanoscale might lead to an effective targeting of new promising bioconversion strategies.

Domaines

Matériaux
Fichier non déposé

Dates et versions

hal-02510434 , version 1 (28-08-2023)

Identifiants

Citer

Eléonore Lambert, Véronique Aguié-Béghin, Delphine Dessaint, Laurence Foulon, Brigitte Chabbert, et al.. Real Time and Quantitative Imaging of Lignocellulosic Films Hydrolysis by Atomic Force Microscopy Reveals Lignin Recalcitrance at Nanoscale. Biomacromolecules, 2019, 20 (1), pp.515-527. ⟨10.1021/acs.biomac.8b01539⟩. ⟨hal-02510434⟩
31 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More