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Communication Dans Un Congrès Année : 2019

Whole brain versus targeted dentate gyrus irradiation model to explain low to moderate doses of exposure effects in mice

Résumé

CT-scan is the most widely used medical diagnostic procedure for pediatric head exploration. Long-term consequences of low to moderate doses of ionizing radiation (IR) exposure during brain ontogeny on behavioral functions are not well established in humans and are a scientific issue of importance. Behavioral functions result from the dynamic interactions of distributed brain areas operating in large-scale networks. The hippocampus is a major cerebral structure involved in behavior, rather in learning and memory for its dorsal and in behavioral response to stress and in emotional and affective functions modulation for its ventral one. The formation of newborn neurons in the sub-granular zone of hippocampal dentate gyrus (DG), all along the life, contribute to these role in behavior. To evaluate IR direct effect on the brain and the role played by DG radiation-induced impairment in behavioral modifications at adult stage, we developed models of brain irradiation and targeted hippocampal dorsal or ventral DG irradiation (doses range 0.25 - 2 Gy) in ten day-old mice (Dos Santos and col., Scie Rep 2018). The technological properties of the high resolution small animal radiation research platform (SARRP) with x-ray tomographic guidance capability were optimized and MR images used. The millimetric ballistic specificity was validated by measuring gamma-H2AX dose dependent-increase after irradiation. Then, anxiety/depressive-like behavior and spatial learning/memory tests were performed at adult stage. Our data suggest : (i) the very high sensitivity of both developing brain and DG to IR, (ii) nonlinear dose effects on memory process after targeted irradiation (effect not observed after total brain exposure) and (iii) learning facilitation in some exposition contexts. This work provides new tools to enhance scientific understanding of how low to moderate doses of IR impact brain and will bring scientific clues for the children radioprotection system.
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hal-02507248 , version 1 (12-03-2020)

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  • HAL Id : hal-02507248 , version 1

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Christelle Durand. Whole brain versus targeted dentate gyrus irradiation model to explain low to moderate doses of exposure effects in mice. International Forum of Neurosciences, FON, Jun 2019, BRUXELLES, Belgium. ⟨hal-02507248⟩
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