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Article Dans Une Revue Journal of Biological Chemistry Année : 2008

New Insights into the Alternative d-Glucarate Degradation Pathway

Asadollah Aghaie
Christophe Lechaplais
Sabine Tricot
  • Fonction : Auteur
  • PersonId : 920749
Véronique de Berardinis
Annett Kreimeyer
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  • PersonId : 920754
Gábor Gyapay
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  • PersonId : 895058
Marcel Salanoubat
Alain Perret

Résumé

characterized in Escherichia coli, genetic and biochemical information concerning the alternative pathway proposed in Pseudomonas species and Bacillus subtilis remains incomplete. Acinetobacter baylyi ADP1 is a Gram-negative soil bacterium possessing the alternative pathway and able to grow using D-glucarate as the only carbon source. Based on the annotation of its sequenced genome (1), we have constructed a complete collection of singlegene deletion mutants (2). High throughput profiling for growth on a minimal medium containing D-glucarate as the only carbon source for 2450 mutants led to the identification of the genes involved in D-glucarate degradation. Protein purification after recombinant production in E. coli allowed us to reconstitute the enzymatic pathway in vitro. We describe here the kinetic characterization of D-glucarate dehydratase, D-5-keto-4-deoxyglucarate dehydratase, and of cooperative -ketoglutarate semialdehyde dehydrogenase. Transcription and expression analyses of the genes involved in D-glucarate metabolism within a single organism made it possible to access information regarding the regulation of this pathway for the first time.

Dates et versions

hal-02503047 , version 1 (09-03-2020)

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Citer

Asadollah Aghaie, Christophe Lechaplais, Peggy Sirven, Sabine Tricot, Marielle Besnard-Gonnet, et al.. New Insights into the Alternative d-Glucarate Degradation Pathway. Journal of Biological Chemistry, 2008, 283 (23), pp.15638-15646. ⟨10.1074/jbc.M800487200⟩. ⟨hal-02503047⟩
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