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Article Dans Une Revue Molecular Systems Biology Année : 2008

A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1

Véronique de Berardinis
David Vallenet
Vanina Castelli
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Christophe Lechaplais
Gábor Gyapay
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Maxime Durot
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  • PersonId : 864084
Annett Kreimeyer
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François Le Fevre
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Vincent Schächter
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Valérie Pezo
Volker Doring
Claude Scarpelli
Claudine Médigue
Philippe Marlière
Marcel Salanoubat
Jean Weissenbach
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  • PersonId : 911249

Résumé

We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a newresource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.

Dates et versions

hal-02503013 , version 1 (09-03-2020)

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Citer

Véronique de Berardinis, David Vallenet, Vanina Castelli, Marielle Besnard, Agnès Pinet, et al.. A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1. Molecular Systems Biology, 2008, 4 (1), pp.174. ⟨10.1038/msb.2008.10⟩. ⟨hal-02503013⟩
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