A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1
Résumé
We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil
bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499
(16%) were not, and are therefore candidate essential genes for life on minimal medium. This
essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio
mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes
described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were
undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our
essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those
from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon
sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a newresource for the study
of gene function by forward and reverse genetic approaches and constitutes a robust experimental
data source for systems biology approaches.