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Article Dans Une Revue Journal of Biomedical Science Année : 2004

Mitochondrial membrane potential (DeltaPsi) and Ca2+-induced differentiation in HaCaT keratinocytes

Mitochondrial membrane potential (DeltaPsi) and Ca(2+)-induced differentiation in HaCaT keratinocytes.

Résumé

We have used the human calcium- and temperature-dependent (HaCaT) keratinocyte cell line to elucidate mechanisms of switching from a proliferating to a differentiating state. When grown in low calcium medium (<0.1 mM) HaCaT cells proliferate. However, an increase in the calcium concentration of the culture medium, [Ca(2+)](0), induces growth arrest and the cells start to differentiate. Numerous studies have already shown that the increase in [Ca(2+)](0) results in acute and sustained increases in intracellular calcium concentration, [Ca(2+)](i). We find that the Ca(2+)-induced cell differentiation of HaCaT cells is also accompanied by a significant decrease in mitochondrial membrane potential, DeltaPsi. By combining patch-clamp electrophysiological recordings and microspectrofluorimetric measurements of DeltaPsi on single cells, we show that the increase in [Ca(2+)](i) led to DeltaPsi depolarization. In addition, we report that tetraethylammonium (TEA), a blocker of plasma membrane K(+) channels, which is known to inhibit cell proliferation, and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), a blocker of plasma membrane Cl(-) channels, also affect DeltaPsi. Both these agents stimulate HaCaT cell differentiation. These data therefore strongly suggest a direct causal relationship between depolarization of DeltaPsi and the inhibition of proliferation and induction of differentiation in HaCaT keratinocytes.
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Dates et versions

hal-02484663 , version 1 (19-02-2020)

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F. Savignan, Bérangère Ballion, M. Odessa, M. Charveron, P. Bordat, et al.. Mitochondrial membrane potential (DeltaPsi) and Ca2+-induced differentiation in HaCaT keratinocytes. Journal of Biomedical Science, 2004, 11 (5), pp.671-682. ⟨10.1007/bf02256133⟩. ⟨hal-02484663⟩

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