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Communication Dans Un Congrès Année : 2020

Pulse-modulated 1800 MHz electromagnetic fields affect microglial cell responses triggered by lipopolysaccharide

Résumé

Mobile communications are propagated by electromagnetic fields (EMFs), and since the 1990s, they operate with pulsemodulated signals such as the GSM-1800 MHz. The biological effects of GSM-EMF in humans affected by neuropathological processes remain seldom investigated. In this study, a 2-h head-only exposure to GSM-1800 MHz was applied to (i) rats undergoing an acute neuroinflammation triggered by a lipopolysaccharide (LPS) treatment, (ii) age-matched healthy rats, or (iii) transgenic hSOD1 G93A rats that modeled a presymptomatic phase of human amyotrophic lateral sclerosis (ALS). Gene responses were assessed 24 h after the GSM head-only exposure in a motor area of the cerebral cortex (mCx) where the mean specific absorption rate (SAR) was estimated to be 3.22 W/kg. In LPS-treated rats, a genome-wide mRNA profiling was performed by RNA-seq analysis and revealed significant (adjusted p value < 0.05) but moderate (fold changes < 2) upregulations or downregulations affecting 2.7% of the expressed genes, including genes expressed predominantly in neuronal or in glial cell types and groups of genes involved in protein ubiquitination or dephosphorylation. Reverse transcription-quantitative PCR analyses confirmed gene modulations uncovered by RNA-seq data and showed that in a set of 15 PCR-assessed genes, significant gene responses to GSM-1800 MHz depended upon the acute neuroinflammatory state triggered in LPS-treated rats, because they were not observed in healthy or in hSOD1 G93A rats. Together, our data specify the extent of cortical gene modulations triggered by GSM-EMF in the course of an acute neuroinflammation and indicate that GSM-induced gene responses can differ according to pathologies affecting the CNS.
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Dates et versions

hal-02465515 , version 1 (16-11-2023)

Identifiants

Citer

Michel Mallat, Julie Lameth, Annie Gervais, Catherine Colin, Philippe Lévêque, et al.. Pulse-modulated 1800 MHz electromagnetic fields affect microglial cell responses triggered by lipopolysaccharide. GLIA Edinburgh 2017, Jul 2017, Edinburgh, United Kingdom. pp.105-123, ⟨10.1007/s12640-020-00191-3⟩. ⟨hal-02465515⟩
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