Cooperation at a distance between silencers and proto-silencers at the yeast HML locus - Archive ouverte HAL
Article Dans Une Revue EMBO Journal Année : 1996

Cooperation at a distance between silencers and proto-silencers at the yeast HML locus

Résumé

Transcriptional repression at the silent yeast mating typelociisachievedthroughtheformationofaparticu- lar nucleoprotein complex at specific cis-acting ele- ments called silencers. This complex in turn appears to initiate the spreading of a histone binding protein complex into the surrounding chromatin, which restrictsaccessibilityoftheregiontothetranscription machinery. We have investigated long-range, cooperative effects between silencers by studying the repressionofa reporter gene integratedattheHML locus flanked by various combinations of wild-type and mutated silencer sequences. Two silencers can cooperate over >4000 bp to repress transcription eficiently.More importantly,a singlebindingsitefor either the repressor activator protein 1 (Rapl), the autonomous replicating sequence (ARS) binding factor 1 (Abfl) or the origin recognition complex (ORC) can enhance the action of a distant silencer without acting as a silencer on its own. Functional cooperativity is demonstratedusingaquantitativeassayforrepression, and varies with the affinity of the binding sites used. Since the repression mechanism is Sir dependent, the Rapl, ORC and/or Abfl proteins bound to distant DNA elements may interact to create an interface of sufficiently high affinity such that Sir-containing complexes bind, nucleating the silent chromatin state.
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Dates et versions

hal-02448173 , version 1 (24-01-2022)

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Cecile Boscheron, Laurent A Maillet, Stephane Marcand, Monika Tsai-Pflugfelder, Susan M Gasser, et al.. Cooperation at a distance between silencers and proto-silencers at the yeast HML locus. EMBO Journal, 1996, 15 (9), pp.2184-2195. ⟨10.1002/j.1460-2075.1996.tb00572.x⟩. ⟨hal-02448173⟩
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