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Article Dans Une Revue BIOMOLECULAR NMR ASSIGNMENTS Année : 2020

1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region

Résumé

The Breast Cancer susceptibility protein 2 (BRCA2) is involved in mechanisms that maintain genome stability, including DNA repair, replication and cell division. These functions are ensured by the folded C-terminal DNA binding domain of BRCA2 but also by its large regions predicted to be disordered. Several studies have shown that disordered regions of BRCA2 are subjected to phosphorylation, thus regulating BRCA2 interactions through the cell cycle. The N-terminal region of BRCA2 contains two highly conserved clusters of phosphorylation sites between amino acids 75 and 210. Upon phosphorylation by CDK, the cluster 1 is known to become a docking site for the kinase PLK1. The cluster 2 is phosphorylated by PLK1 at least at two positions. Both of these phosphorylation clusters are important for mitosis progression, in particular for chromosome segregation and cytokinesis. In order to identify the phosphorylated residues and to characterize the phosphorylation sites preferences and their functional consequences within BRCA2 N-terminus, we have produced and analyzed the BRCA2 fragment from amino acid 48 to amino acid 284 (BRCA248-284). Here, we report the assignment of 1H, 15N, 13CO, 13Cα and 13Cβ NMR chemical shifts of this region. Analysis of these chemical shifts confirmed that BRCA248-284 shows no stable fold: it is intrinsically disordered, with only short, transient α-helices.
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Dates et versions

hal-02445473 , version 1 (07-12-2020)

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Manon Julien, Simona Miron, Aura Carreira, François-Xavier Theillet, Sophie Zinn-Justin. 1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region. BIOMOLECULAR NMR ASSIGNMENTS, 2020, 14 (1), pp.79-85. ⟨10.1007/s12104-019-09924-8⟩. ⟨hal-02445473⟩
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