Dynamin-2 regulates fusion pore expansion and quantal release through a mechanism that involves actin dynamics in neuroendocrine chromaffin cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2013

Dynamin-2 regulates fusion pore expansion and quantal release through a mechanism that involves actin dynamics in neuroendocrine chromaffin cells.

Arlek M González-Jamett
  • Fonction : Auteur
Fanny Momboisse
  • Fonction : Auteur
María José Guerra
  • Fonction : Auteur
Ximena Báez-Matus
  • Fonction : Auteur
Natalia Barraza
  • Fonction : Auteur
Valerie Calco
  • Fonction : Auteur
Eduardo Couve
  • Fonction : Auteur
Alan Neely
  • Fonction : Auteur
Agustín D Martínez
  • Fonction : Auteur
Stephane Gasman
Ana M Cárdenas
  • Fonction : Auteur

Résumé

Over the past years, dynamin has been implicated in tuning the amount and nature of transmitter released during exocytosis. However, the mechanism involved remains poorly understood. Here, using bovine adrenal chromaffin cells, we investigated whether this mechanism rely on dynamin's ability to remodel actin cytoskeleton. According to this idea, inhibition of dynamin GTPase activity suppressed the calcium-dependent de novo cortical actin and altered the cortical actin network. Similarly, expression of a small interfering RNA directed against dynamin-2, an isoform highly expressed in chromaffin cells, changed the cortical actin network pattern. Disruption of dynamin-2 function, as well as the pharmacological inhibition of actin polymerization with cytochalasine-D, slowed down fusion pore expansion and increased the quantal size of individual exocytotic events. The effects of cytochalasine-D and dynamin-2 disruption were not additive indicating that dynamin-2 and F-actin regulate the late steps of exocytosis by a common mechanism. Together our data support a model in which dynamin-2 directs actin polymerization at the exocytosis site where both, in concert, adjust the hormone quantal release to efficiently respond to physiological demands.
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Dates et versions

hal-02440089 , version 1 (14-01-2020)

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Arlek M González-Jamett, Fanny Momboisse, María José Guerra, Stephane Ory, Ximena Báez-Matus, et al.. Dynamin-2 regulates fusion pore expansion and quantal release through a mechanism that involves actin dynamics in neuroendocrine chromaffin cells.. PLoS ONE, 2013, 8 (8), pp.e70638. ⟨10.1371/journal.pone.0070638⟩. ⟨hal-02440089⟩

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