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Article Dans Une Revue Journal of Pharmaceutical and Biomedical Analysis Année : 2019

Validated analytical method to determine new salivary lipid peroxidation compounds as potential neurodegenerative biomarkers

Résumé

Lipid peroxidation is closely related to neurodegenerative diseases since brain shows high lipid composition and oxygen consumption. The determination of lipid peroxidation compounds in non-invasive biological samples would help to monitor the patients' oxidative stress status. A new analytical method based on ultrasound-assisted liquid-liquid semi-microextraction (UA-LLsME) followed by Ultra Performance Liquid Chromatography coupled to tandem Mass Spectrometry was developed to determine 18 lipid peroxidation biomarkers in saliva samples. The variables affecting the UA-LLsME efficiency were systematically studied. Under the optimum conditions, the methodology was validated and showed high-throughput, high sensitivity (limits of detection 0.02-2 nmol L −1), and satisfactory precision (coef-ficients of variation 2-11% (intra-day) and 5-12% (inter-day)). The reliability of the described method was assessed analysing spiked saliva samples, and the recoveries were between 80% and 120% for most of the analytes. Then, the method suitability was demonstrated by analysing saliva samples (n = 30) from elderly people with neurodegenerative diseases. To conclude, the new developed analytical method is a useful tool to determine salivary lipid peroxidation compounds as potential biomarkers in further clinical studies in which oxidative stress plays an important role.

Domaines

Chimie
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Dates et versions

hal-02417284 , version 1 (18-12-2019)

Identifiants

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Carmen Peña-Bautista, Paula Carrascosa-Marco, Camille Oger, Claire Vigor, Jean-Marie Galano, et al.. Validated analytical method to determine new salivary lipid peroxidation compounds as potential neurodegenerative biomarkers. Journal of Pharmaceutical and Biomedical Analysis, 2019, 164, pp.742-749. ⟨10.1016/j.jpba.2018.11.043⟩. ⟨hal-02417284⟩
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