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Preprints, Working Papers, ... Year : 2019

GC content shapes mRNA decay and storage in human cells

Maïte Courel
Dominika Foretek
  • Function : Author
Olivia Vidal Cruchez
  • Function : Author
Zhou Yi
  • Function : Author
Marie-Noëlle Benassy
  • Function : Author
Michel Kress
  • Function : Author
Caroline Vindry
  • Function : Author
Marianne Bénard
  • Function : Author
Clémentine Bossevain
  • Function : Author
Christophe Antoniewski
Antonin Morillon
Patrick Brest
Arnaud Hubstenberger
  • Function : Author
Hugues Roest Crollius
  • Function : Author
Nancy Standart
Dominique Weil


Control of protein expression results from the fine tuning of mRNA synthesis, decay and translation. These processes, which are controlled by a large number of RNA-binding proteins and by localization in RNP granules such as P-bodies, appear often intimately linked although the rules of this interplay are not well understood. In this study, we combined our recent P-body transcriptome with various transcriptomes obtained following silencing of broadly acting mRNA decay and repression factors. This analysis revealed the central role of GC content in mRNA fate, in terms of P-body localization, mRNA translation and mRNA decay. It also rationalized why PBs mRNAs have a strikingly low protein yield. We report too the existence of distinct mRNA decay pathways with preference for AU-rich or GC-rich transcripts. Compared to this impact of the GC content, sequence-specific RBPs and miRNAs appeared to have only modest additional effects on their bulk targets. Altogether, these results lead to an integrated view of post-transcriptional control in human cells where most regulation at the level of translation is dedicated to AU-rich mRNAs, which have a limiting protein yield, whereas regulation at the level of 5' decay applies to GC-rich mRNAs, whose translation is optimal.
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Dates and versions

hal-02415568 , version 1 (17-12-2019)



Maïte Courel, Yves Clément, Dominika Foretek, Olivia Vidal Cruchez, Zhou Yi, et al.. GC content shapes mRNA decay and storage in human cells. 2019. ⟨hal-02415568⟩
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