Ets-1 drives breast cancer cell angiogenic potential and interactions between breast cancer and endothelial cells - Archive ouverte HAL
Article Dans Une Revue International Journal of Oncology Année : 2018

Ets-1 drives breast cancer cell angiogenic potential and interactions between breast cancer and endothelial cells

Résumé

Ets-1 transcription factor overexpression in breast cancers is associated with invasive features and is associated with a poor prognosis. Beyond its role in driving carcinoma cell invasion, in this study, we wished to determine whether Ets-1 overexpression in cancer cells promotes angiogenesis by creating a paracrine pro-invasive environment for endothelial cells as well. To address this question, we set up different co-culture models of cancer cells with endothelial cells. Conditioned media from cancer cells induced endothelial cell proliferation, migration and morphogenesis in matrix models. Of note, co-culture assays in three-dimensional matrix models also revealed the reciprocal induction of cancer cell morphogenesis by endothelial cells, in support of an angiocrine action on tumor cells. Ets-1 emerged as a key regulator of the angiogenic potential of breast cancer cells, favoring their ability to induce, in a paracrine manner, the morphogenesis of endothelial cells and also to physically interact with the latter. Nevertheless, Ets-1 overexpression in cancer cells also restrained their chemoattractive potential for endothelial cells both in Boyden chambers and in ex vivo 3D co-cultures. Finally, Ets-1 modulation in breast cancer cells qualitatively altered the angiogenic pattern of experimental in vivo tumors, with a balance between vessel recruitment and intratumoral small capillaries sprouting. Taken together, our data highlight a critical and intriguing role for Ets-1 in the angiogenic potential of breast cancer cells, and reveal another facet of Ets-1 oncogenic activities.
Fichier principal
Vignette du fichier
ijo_54_1_29_PDF.pdf (1.99 Mo) Télécharger le fichier
Origine Publication financée par une institution
Loading...

Dates et versions

hal-02413908 , version 1 (23-12-2019)

Licence

Identifiants

Citer

Alessandro Furlan, Chantal Vercamer, Laurent Héliot, Nicolas Wernert, Xavier Desbiens, et al.. Ets-1 drives breast cancer cell angiogenic potential and interactions between breast cancer and endothelial cells. International Journal of Oncology, 2018, 54 (1), pp.29-40. ⟨10.3892/ijo.2018.4605⟩. ⟨hal-02413908⟩
56 Consultations
34 Téléchargements

Altmetric

Partager

More