Article Dans Une Revue Autophagy Année : 2015

Guidelines for monitoring autophagy in Caenorhabditis elegans

Qun Lu
  • Fonction : Auteur
Alicia Meléndez
  • Fonction : Auteur
Eyleen J. O'Rourke
  • Fonction : Auteur
Ken Sato
  • Fonction : Auteur
Miyuki Sato
  • Fonction : Auteur
Xiaochen Wang
  • Fonction : Auteur
Fan Wu
  • Fonction : Auteur
Hong Zhang
  • Fonction : Auteur
Jessica T. Chang
  • Fonction : Auteur
Bin Guo
Malene Hansen
  • Fonction : Auteur
Kailiang Jia
  • Fonction : Auteur
Attila L. Kovács
  • Fonction : Auteur
Caroline Kumsta
  • Fonction : Auteur
Louis R. Lapierre
  • Fonction : Auteur
Long Lin
  • Fonction : Auteur

Résumé

The cellular recycling process of autophagy has been extensively characterized with standard assays in yeast and mammalian cell lines. In multicellular organisms, numerous external and internal factors differentially affect autophagy activity in specific cell types throughout the stages of organismal ontogeny, adding complexity to the analysis of autophagy in these metazoans. Here we summarize currently available assays for monitoring the autophagic process in the nematode C. elegans. A combination of measuring levels of the lipidated Atg8 ortholog LGG-1, degradation of well-characterized autophagic substrates such as germline P granule components and the SQSTM1/p62 ortholog SQST-1, expression of autophagic genes and electron microscopy analysis of autophagic structures are presently the most informative, yet steady-state, approaches available to assess autophagy levels in C. elegans. We also review how altered autophagy activity affects a variety of biological processes in C. elegans such as L1 survival under starvation conditions, dauer formation, aging, and cell death, as well as neuronal cell specification. Taken together, C. elegans is emerging as a powerful model organism to monitor autophagy while evaluating important physiological roles for autophagy in key developmental events as well as during adulthood.

Dates et versions

hal-02400424 , version 1 (09-12-2019)

Identifiants

Citer

Qun Lu, Alicia Meléndez, Eyleen J. O'Rourke, Ken Sato, Miyuki Sato, et al.. Guidelines for monitoring autophagy in Caenorhabditis elegans. Autophagy, 2015, 11 (1), pp.9--27. ⟨10.1080/15548627.2014.1003478⟩. ⟨hal-02400424⟩
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