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Article Dans Une Revue Nature Communications Année : 2019

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors

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Cell-free transcription–translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media, including commercial beverages and human urine, in which they can be used to detect clinically relevant concentrations of small molecules. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.
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hal-02399479 , version 1 (26-05-2020)

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Peter Voyvodic, Amir Pandi, Mathilde Koch, Ismael Conejero, Emmanuel Valjent, et al.. Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors. Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-09722-9⟩. ⟨hal-02399479⟩
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