The new unsymmetrical indene-based pro-ligand featuring thiophosphinoyle and methylpyridine sidearms 2 was prepared. Coordination and cyclometalation in the presence of [PdCl2(PhCN)2] and PS-DIEA afforded three well-defined 2-indenyl SCN pincer complexes 3a-c. The lability of the pyridine moiety has been evidenced upon treatment with triphenylphosphine and 2,6-dimethylphenylisocyanide. In addition, reversible C-Pd bond cleavage has been demonstrated under Brönsted acid/base conditions. The indenediide SCN pincer complex 4 was prepared by deprotonation of 3a in the presence of triphenylphosphine. Preliminary catalytic tests on the cyloisomerization of 4-pentynoic acid have underlined the impact of the pyridine sidearm on catalytic activity.