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Article Dans Une Revue Science Année : 2019

Active cell migration is critical for steady-state epithelial turnover in the gut

Denis Krndija
Fatima El Marjou
  • Fonction : Auteur
Boris Guirao
Olivier Leroy
  • Fonction : Auteur
  • PersonId : 919212
Edouard Hannezo
  • Fonction : Auteur
  • PersonId : 980206
Danijela Matic Vignjevic
  • Fonction : Auteur

Résumé

Steady-state turnover is a hallmark of epithelial tissues throughout adult life. Intestinal epithelial turnover is marked by continuous cell migration, which is assumed to be driven by mitotic pressure from the crypts. However, the balance of forces in renewal remains ill-defined. Combining biophysical modeling and quantitative three-dimensional tissue imaging with genetic and physical manipulations, we revealed the existence of an actin-related protein 2/3 complex-dependent active migratory force, which explains quantitatively the profiles of cell speed, density, and tissue tension along the villi. Cells migrate collectively with minimal rearrangements while displaying dual-apicobasal and front-back-polarity characterized by actin-rich basal protrusions oriented in the direction of migration. We propose that active migration is a critical component of gut epithelial turnover.
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Dates et versions

hal-02397547 , version 1 (06-12-2019)

Identifiants

  • HAL Id : hal-02397547 , version 1

Citer

Denis Krndija, Fatima El Marjou, Boris Guirao, Sophie Richon, Olivier Leroy, et al.. Active cell migration is critical for steady-state epithelial turnover in the gut. Science, 2019. ⟨hal-02397547⟩
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