Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2019

Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability

Résumé

In the soil-dwelling organism Pseudomonas putida KT2440, the rare earth element (REE)-utilizing, and pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase PedH is part of a periplasmic oxidation system that is vital for growth on various alcoholic volatiles. Production of PedH and its Ca 2+-dependent counterpart PedE is inversely regulated in response to lanthanide (Ln 3+) bioavailability, a mechanism termed the REEswitch. In the present study, we demonstrate that copper, zinc, and in particular, iron availability influences this regulation in a pyoverdine-independent manner by increasing the minimal Ln 3+ concentration required for the REE-switch to occur by several orders of magnitude. A combined genetic and physiological approach reveals that an ABC-type transporter system encoded by the gene cluster pedA1A2BC is essential for efficient growth on 2-phenylethanol with low (nanomolar) Ln 3+ concentrations. In the absence of pedA1A2BC, a ∼100-fold higher La 3+-concentration is needed for PedH-dependent growth but not for the ability to repress growth based on PedE activity. From these results, we conclude that cytoplasmic uptake of lanthanides through PedA1A2BC is essential to facilitate REE-dependent growth on 2-phenylethanol under environmental conditions with poor REE bioavailability. Our data further suggest that the La 3+ /Fe 2+/3+ ratio impacts the REE-switch through the mismetallation of putative La 3+-binding proteins, such as the sensor histidine kinase PedS2, in the presence of high iron concentrations. As such, this study provides an example for the complexity of bacteria-metal interactions and highlights the importance of medium compositions when studying physiological traits in vitro in particular in regard to REE-dependent phenomena.
Fichier principal
Vignette du fichier
fmicb-10-02494.pdf (1.94 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02389228 , version 1 (08-01-2021)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Matthias Wehrmann, Charlotte Berthelot, Patrick Billard, Janosch Klebensberger. Rare Earth Element (REE)-Dependent Growth of Pseudomonas putida KT2440 Relies on the ABC-Transporter PedA1A2BC and Is Influenced by Iron Availability. Frontiers in Microbiology, 2019, 10, ⟨10.3389/fmicb.2019.02494⟩. ⟨hal-02389228⟩
182 Consultations
118 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More