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Chapitre D'ouvrage Année : 2018

Stable Inactivation of MicroRNAs in Medicago truncatula Roots

Résumé

MicroRNAs are key regulators in the development processes or stress responses in plants. In the last decade, several conserved or non-conserved microRNAs have been identified in Medicago truncatula. Different strategies leading to the inactivation of microRNAs in plants have been described. Here, we propose a protocol for an effective inactivation of microRNAs using a STTM strategy in M. truncatula transgenic roots.
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Dates et versions

hal-02383697 , version 1 (27-11-2019)

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Hélène Proust, Jérémie Bazin, Céline Sorin, Caroline Hartmann, Martin Crespi, et al.. Stable Inactivation of MicroRNAs in Medicago truncatula Roots. Methods in Molecular Biology, 1822, Humana Press Inc., pp.123-132, 2018, Methods in Molecular Biology, 978-1-4939-8633-0; 978-1-4939-8632-3. ⟨10.1007/978-1-4939-8633-0_9⟩. ⟨hal-02383697⟩
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