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Article Dans Une Revue Genome Biology Année : 2019

CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Haroon Butt
  • Fonction : Auteur
Ayman Eid
  • Fonction : Auteur
Afaque Momin
  • Fonction : Auteur
Magdy Mahfouz
  • Fonction : Auteur

Résumé

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions.
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hal-02383690 , version 1 (26-05-2020)

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Haroon Butt, Ayman Eid, Afaque Momin, Jérémie Bazin, Martin Crespi, et al.. CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors. Genome Biology, 2019, 20, pp.73. ⟨10.1186/s13059-019-1680-9⟩. ⟨hal-02383690⟩
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