A numerical model suggests the interplay between nuclear plasticity and stiffness during a perfusion assay - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Theoretical Biology Année : 2017

A numerical model suggests the interplay between nuclear plasticity and stiffness during a perfusion assay

Résumé

Cell deformability is a necessary condition for a cell to be able to migrate, an ability that is vital both for healthy and diseased organisms. The nucleus being the largest and stiffest organelle, it often is a barrier to cell migration. It is thus essential to characterize its mechanical behaviour. First, we numerically investigate the visco-elasto-plastic properties of the isolated nucleus during a compression test. This simulation highlights the impact of the mechanical behaviour of the nuclear lamina and the nucleoplasm on the overall plasticity. Second, a whole cell model is developed to simulate a perfusion experiment to study the possible interactions between the cytoplasm and the nucleus. We analyze and discuss the role of the lamina for a wild-type cell model, and a lamin-deficient one, in which the Young’s modulus of the lamina is set to 1% of its nominal value. This simulation suggests an interplay between the cytoplasm and the nucleoplasm, especially in the lamin-deficient cell, showing the need of a stiffer nucleoplasm to maintain nuclear plasticity.
Fichier principal
Vignette du fichier
IBHGC_JTB_2017_ALLENA.pdf (2.83 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02379089 , version 1 (25-11-2019)

Identifiants

Citer

Solenne Deveraux, Rachele Allena, Denis Aubry. A numerical model suggests the interplay between nuclear plasticity and stiffness during a perfusion assay. Journal of Theoretical Biology, 2017, 435, pp.62-77. ⟨10.1016/j.jtbi.2017.09.007⟩. ⟨hal-02379089⟩
57 Consultations
84 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More