A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2014

A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase

Résumé

The Plasmodium subtilase SUB1 plays a pivotal role during the egress of malaria parasites from host hepatocytes and erythrocytes. Here we report the crystal structure of full-length SUB1 from the human-infecting parasite Plasmodium vivax, revealing a bacterial-like catalytic domain in complex with a Plasmodium-specific prodomain. The latter displays a novel architecture with an amino-terminal insertion that functions as a 'belt', embracing the catalytic domain to further stabilize the quaternary structure of the pre-protease, and undergoes calcium-dependent autoprocessing during subsequent activation. Although dispensable for recombinant enzymatic activity, the SUB1 'belt' could not be deleted in Plasmodium berghei, suggesting an essential role of this domain for parasite development in vivo. The SUB1 structure not only provides a valuable platform to develop new anti-malarial candidates against this promising drug target, but also defines the Plasmodium-specific 'belt' domain as a key calcium-dependent regulator of SUB1 during parasite egress from host cells.

Domaines

Parasitologie

Dates et versions

hal-02378692 , version 1 (25-11-2019)

Identifiants

Citer

David Giganti, Anthony Bouillon, Lina Tawk, Fabienne Robert, Mariano Martinez, et al.. A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase. Nature Communications, 2014, 5 (1), ⟨10.1038/ncomms5833⟩. ⟨hal-02378692⟩
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