The EUREQUA project raises the issue of the definition and evaluation of the environmental quality of neighbourhoods. The approach consists of integrating and cross-referencing observable data characterising the physical environment and people's perception of their quality of life. The study area is a neighbourhood in Toulouse (France) with high social and typo-morphological diversity, subject to noise and air pollution nuisances. Three 3-day field campaigns were organised in January, April, and June 2014. Instrumented and commented walks took place three times per day. For each one, measurements of physical environmental parameters and surveys were performed simultaneously at six locations in the neighbourhood. The study focuses on microclimate and thermal comfort issues. It aims to compare in situ meteorological data of air temperature, humidity, wind speed, and mean radiant temperature, with quantitative results rating human perception of heat, humidity, wind, and thermal comfort. The variability in perception and measurements is mainly driven by seasonal effects, especially for heat and humidity, and, to a lesser extent, for wind. Wind perception and measurement also vary spatially, thus highlighting site effects. Linear models indicate a positive link between heat perception and mean radiant temperature, as well as between wind perception and mean and standard deviation of wind speed (with a higher sensitivity of people to wind under winter climate conditions). Finally, it is found that perception of thermal comfort is only slightly linked to the different microclimate dimensions, and is rather driven by other appreciation factors and emotional criteria related to the general environmental quality of the study area.