Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2018

Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1

Résumé

We previously demonstrated that the translocase protein TSPO2 together with the voltage-dependent anion channel (VDAC) and adenine nucleotide transporter (ANT) were involved in a membrane transport complex in human red blood cells (RBCs). Because VDAC was proposed as a channel mediating ATP release in RBCs, we used TSPO ligands together with VDAC and ANT inhibitors to test this hypothesis. ATP release was activated by TSPO ligands, and blocked by inhibitors of VDAC and ANT, while it was insensitive to pannexin-1 blockers. TSPO ligand increased extracellular ATP (ATPe) concentration by 24–59% over the basal values, displaying an acute increase in [ATPe] to a maximal value, which remained constant thereafter. ATPe kinetics were compatible with VDAC mediating a fast but transient ATP efflux. ATP release was strongly inhibited by PKC and PKA inhibitors as well as by depleting intracellular cAMP or extracellular Ca2+, suggesting a mechanism involving protein kinases. TSPO ligands favoured VDAC polymerization yielding significantly higher densities of oligomeric bands than in unstimulated cells. Polymerization was partially inhibited by decreasing Ca2+ and cAMP contents. The present results show that TSPO ligands induce polymerization of VDAC, coupled to activation of ATP release by a supramolecular complex involving VDAC, TSPO2 and ANT
Fichier principal
Vignette du fichier
s41598-018-29885-7.pdf (1.77 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02368694 , version 1 (26-10-2021)

Identifiants

Citer

Irene Marginedas-Freixa, Cora Lilia Alvarez, Martina Moras, María Florencia Leal Denis, Claude Hattab, et al.. Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1. Scientific Reports, 2018, 8, pp.11384. ⟨10.1038/s41598-018-29885-7⟩. ⟨hal-02368694⟩
39 Consultations
29 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More