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Article Dans Une Revue PLoS ONE Année : 2012

Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis

Résumé

Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.
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Dates et versions

hal-02368517 , version 1 (18-11-2019)

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C. Nogues, H Leh, J. Lautru, O. Delelis, Malcolm Buckle. Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis. PLoS ONE, 2012, 7, ⟨10.1371/journal.pone.0044287⟩. ⟨hal-02368517⟩
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