High-resolution biophysical analysis of the dynamics of nucleosome formation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2016

High-resolution biophysical analysis of the dynamics of nucleosome formation

Résumé

We describe a biophysical approach that enables changes in the structure of DNA to be followed during nucleosome formation in in vitro reconstitution with either the canonical “Widom” sequence or a judiciously mutated sequence. The rapid non-perturbing photochemical analysis presented here provides ‘snapshots’ of the DNA configuration at any given moment in time during nucleosome formation under a very broad range of reaction conditions. Changes in DNA photochemical reactivity upon protein binding are interpreted as being mainly induced by alterations in individual base pair roll angles. The results strengthen the importance of the role of an initial (H3/H4)2 histone tetramer-DNA interaction and highlight the modulation of this early event by the DNA sequence. (H3/H4)2 binding precedes and dictates subsequent H2A/H2B-DNA interactions, which are less affected by the DNA sequence, leading to the final octameric nucleosome. Overall, our results provide a novel, exciting way to investigate those biophysical properties of DNA that constitute a crucial component in nucleosome formation and stabilization.
Fichier principal
Vignette du fichier
Nature Publishing Group 2016 Hatakeyama.pdf (1.78 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02368359 , version 1 (18-11-2019)

Identifiants

Citer

Akiko Hatakeyama, Brigitte Hartmann, Andrew Travers, Claude Nogues, Malcolm Buckle. High-resolution biophysical analysis of the dynamics of nucleosome formation. Scientific Reports, 2016, 6, pp.27337. ⟨10.1038/srep27337⟩. ⟨hal-02368359⟩
59 Consultations
40 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More