Photochemical analysis of structural transitions in DNA liquid crystals reveals differences in spatial structure of DNA molecules organized in liquid crystalline form - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2018

Photochemical analysis of structural transitions in DNA liquid crystals reveals differences in spatial structure of DNA molecules organized in liquid crystalline form

Résumé

The anisotropic shape of DNA molecules allows them to form lyotropic liquid crystals (LCs) at high concentrations. This liquid crystalline arrangement is also found in vivo (e.g., in bacteriophage capsids, bacteria or human sperm nuclei). However, the role of DNA liquid crystalline organization in living organisms still remains an open question. Here we show that in vitro, the DNA spatial structure is significantly changed in mesophases compared to non-organized DNA molecules. DNA LCs were prepared from pBluescript SK (pBSK) plasmid DNA and investigated by photochemical analysis of structural transitions (PhAST). We reveal significant differences in the probability of UV-induced pyrimidine dimer photoproduct formation at multiple loci on the DNA indicative of changes in major groove architecture.
Fichier principal
Vignette du fichier
Brach_et_al-2018-Scientific_Reports.pdf (3.28 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02368336 , version 1 (18-11-2019)

Identifiants

Citer

Katarzyna Brach, Akiko Hatakeyama, Claude Nogues, Joanna Olesiak-Banska, Malcolm Buckle, et al.. Photochemical analysis of structural transitions in DNA liquid crystals reveals differences in spatial structure of DNA molecules organized in liquid crystalline form. Scientific Reports, 2018, 8, ⟨10.1038/s41598-018-22863-z⟩. ⟨hal-02368336⟩
22 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More