A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGF alpha-Mediated Recruitment of Neutrophils
Baptiste Janela
,
Amit A. Patel
,
Mai Chan Lau
,
Chi Ching Goh
,
Rasha Msallam
(1, 2)
,
Wan Ting Kong
,
Michael Fehlings
(3)
,
Sandra Hubert
(4)
,
Josephine Lum
(4, 1)
,
Yannick Simoni
(5)
,
Benoit Malleret
(6, 4)
,
Francesca Zolezzi
(7)
,
Jinmiao Chen
(4)
,
Michael Poidinger
(4, 1)
,
Ansuman T. Satpathy
,
Carlos Briseno
(8)
,
Christian Wohn
(9)
,
Bernard Malissen
(9)
,
Kenneth M. Murphy
,
Alexander A. Maini
,
Leen Vanhoutte
,
Martin Guilliams
(10)
,
Emmanuel Vial
(11)
,
Laurent Hennequin
(12)
,
Evan Newell
,
Lai Guan Ng
,
Philippe Musette
(13)
,
Simon Yona
,
Feriel Hacini-Rachinel
(14)
,
Florent Ginhoux
(1)
1
A*STAR -
Agency for science, technology and research [Singapore]
2 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
3 University of Toronto
4 SIgN - Singapore Immunology Network
5 IC UM3 (UMR 8104 / U1016) - Institut Cochin
6 Yong Loo Lin School of Medicine - Department of Microbiology & Immunology [Singapore]
7 Department of Biotechnology and Biosciences
8 Washington University School of Medicine in St. Louis
9 CIML - Centre d'Immunologie de Marseille - Luminy
10 UGENT - Universiteit Gent = Ghent University = Université de Gand
11 Virologie humaine
12 Nestle Skin Health GALDERMA R&D
13 Service de Dermatologie [Rouen]
14 Galderma International S.A.S. [Paris, France]
2 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
3 University of Toronto
4 SIgN - Singapore Immunology Network
5 IC UM3 (UMR 8104 / U1016) - Institut Cochin
6 Yong Loo Lin School of Medicine - Department of Microbiology & Immunology [Singapore]
7 Department of Biotechnology and Biosciences
8 Washington University School of Medicine in St. Louis
9 CIML - Centre d'Immunologie de Marseille - Luminy
10 UGENT - Universiteit Gent = Ghent University = Université de Gand
11 Virologie humaine
12 Nestle Skin Health GALDERMA R&D
13 Service de Dermatologie [Rouen]
14 Galderma International S.A.S. [Paris, France]
Baptiste Janela
- Fonction : Auteur
Amit A. Patel
- Fonction : Auteur
Mai Chan Lau
- Fonction : Auteur
Chi Ching Goh
- Fonction : Auteur
Wan Ting Kong
- Fonction : Auteur
Josephine Lum
- Fonction : Auteur
- PersonId : 794962
- ORCID : 0000-0003-1108-5989
Michael Poidinger
- Fonction : Auteur
- PersonId : 773545
- ORCID : 0000-0002-1047-2277
Ansuman T. Satpathy
- Fonction : Auteur
Bernard Malissen
- Fonction : Auteur
- PersonId : 739259
- IdHAL : bernard-malissen
- IdRef : 03049690X
Kenneth M. Murphy
- Fonction : Auteur
Alexander A. Maini
- Fonction : Auteur
Leen Vanhoutte
- Fonction : Auteur
Evan Newell
- Fonction : Auteur
Lai Guan Ng
- Fonction : Auteur
Simon Yona
- Fonction : Auteur
Florent Ginhoux
- Fonction : Auteur
- PersonId : 759995
- ORCID : 0000-0002-2857-7755
Résumé
Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor alpha (VEGF-alpha) by a minor subset of activated EpCAM(+)CD59(+) Ly-6D(+) cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guerin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.